Reading a Certificate of Analysis: A Researcher’s Guide to Peptide Purity

A Certificate of Analysis (CoA) is a document issued by an independent, accredited laboratory confirming the identity, purity, and batch-specific characteristics of a compound. For research peptides, the CoA is the primary quality document — it is the researcher’s assurance that what is labeled on the vial corresponds to what is actually inside. Biotidez provides a CoA for every product, available for download directly on each product page.

The most important sections to review are: Identity confirmation (typically via HPLC-MS or mass spectrometry, confirming the molecular weight matches the expected value for the peptide sequence); Purity percentage (reported as area-under-curve percentage from HPLC — Biotidez products are verified at >99%); Appearance (lyophilized peptides should be a white to off-white powder); and Residual solvents and heavy metals (if tested — relevant for compounds intended for sensitive assay environments).

When reviewing a CoA, pay attention to the testing laboratory’s accreditation status and the test date relative to the batch date. A CoA from a non-independent (in-house) laboratory carries less evidentiary weight than one from a third-party facility operating under ISO/IEC 17025 or equivalent standards. All Biotidez CoAs are generated by independent third-party laboratories and are batch-specific — not generic documents applied across multiple lots.

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